p53 monoclonal antibody

The best p53 monoclonal antibody, quick delivery, high quality.

Product price

111 EUR

Product size

25ug

Product catalog no.

11-114-C025

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Background

The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.

References

*Dolezalova H, Vojtesek B, Kovarik J: Epitope analysis of the human p53 tumour suppressor protein. Folia Biol (Praha). 1997;43(1):49-51. _x000D_ , *Bartkova J, Bartek J, Lukas J, Vojtesek B, Staskova Z, Rejthar A, Kovarik J, Midgley CA, Lane DP: p53 protein alterations in human testicular cancer including pre-invasive intratubular germ-cell neoplasia. Int J Cancer. 1991 Sep 9;49(2):196-202. _x000D_ , *Bartek J, Bartkova J, Vojtesek B, Staskova Z, Lukas J, Rejthar A, Kovarik J, Midgley CA, Gannon JV, Lane DP: Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies. Oncogene. 1991 Sep;6(9):1699-703. _x000D_

 

Shipping and storage

Mouse Monoclonal Antibody against Human p53, Clone: BP53-12 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

 

Specificity

The antibody BP53-12 recognizes defined epitope (aa 16-25) on human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells; it is frequently mutated or inactivated in many types of cancer.

Research area

Transcription Regulation & Nuclear Proteins (Veterinary), Transcription Regulation & Nuclear Proteins (Human), Transcription Regulation & Nuclear Proteins

 

Negative species reactivity

at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity

Properties

If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

 

Storage buffer

Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Long name

Mouse Monoclonal Antibody against Human p53, Clone: BP53-12

 

Species reactivity

Human (Homo sapiens), Non-Human (Homo sapiens) Primates

Immunogen

Bacterially expressed full-length wild-type p53

 

Purification method

Purified by precipitation methods

Applications

FC, IP, WB, IHC(P), ICC, ELISA

 

Clonality

Monoclonal Antibody (Mab)

Format/conjugation

purified; unconjugated

 

Purity

> 95% (by SDS-PAGE)

French translation

anticorps

 

Concentration

1 mg/ml

Clone

BP53-12

 

Antigen

p53