Mouse anti p53 (a.a. 371-380)

The best Mouse anti p53 (a.a. 371-380), quick delivery, high quality.

Product price

304 EUR

Product size

100 μg

Product catalog no.

X1493M

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Long description

p53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.

Relevant references

1. Dubel, S.J. et.al. 'Molecular cloning of the alpha-1 subunit of an omega-conotoxin-sensitive calcium channel' Proc. Natl. Acad. Sci. U.S.A. 89 (11), 5058-5062 (1992)_x000B__x000B_2. Snutch,T.P.et.al. 'Rat brain expresses a heterogeneous family of calcium channels' Proc. Natl. Acad. Sci. U.S.A. 87 (9), 3391-3395 (1990)_x000B__x000B_3. Hell, J.W. et.al. 'Differential phosphorylation of two size forms of the N-type calcium channel alpha 1 subunit which have different COOH termini' J. Biol. Chem. 269 (10), 7390-7396 (1994)_x000B__x000B_4. Sunan, A.A. et.al. 'The C terminus of the Ca channel alpha1B subunit mediates selective inhibition by G-protein-coupled receptors.' J Neurosci. 21(19), 7587-97. (2001)

 

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

 

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Antibody's suited for

This antibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.

 

Other description

Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography

Antibody come from

Synthetic peptide derived from the rat alpha1B calcium channel conjugated to KLH

 

Application

ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry

Antibody's reacts with these species

This antibody doesn't cross react with other species

 

Antigen-antibody binding interaction

Mouse anti p53 (a.a. 371-380) Antibody

Antibody's reacts with

Human, Monkey, Mouse, Rat

 

Category

Primary Antibodies

Antibody's specificity

No Data Available

 

Latin name

Mus musculus

Protein number

see ncbi

 

Storage

-20ºC

Antibody is raised in

Mouse

 

Clone

HR231