Rabbit anti PIG3 (p53 inducible gene 3)

The best Rabbit anti PIG3 (p53 inducible gene 3), quick delivery, high quality.

Product price

304 EUR

Product size

100 μg

Product catalog no.

X1155P

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Long description

p53 inducible gene-3 (PIG-3) may be a long-lived reporter, which may be useful for detecting transient activation of p53. The p53 tumor suppressor is the most commonly mutated gene in human cancers. The p53 protein, which is stabilized in response to different biological checkpoints, is activated by DNA damage, hypoxia, viral infection, or oncogene activation resulting in efects such as cell cycle arrest, apoptosis, senescence, differentiation, and antiangiogenesis. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others. The p53-inducible gene 3 (PIG3), was recently identified in a screen for genes induced by p53 before the onset of apoptosis. PIG3 shares significant homology with the oxidoreductases from several species. PIG3 protein is localized to the cytoplasm and induced in primary, non-transformed, and transformed cell cultures after exposure to genotoxic agents. The induction of PIG3 by p53 occurrs with delayed kinetics as compared with other p53 downstream targets, such as p21 and MDM2. PIG3 levels are increased during p53-mediated growth arrest. Elevated levels of PIG3 are maintained in cells that resume cycling in the absence of ectopic p53 expression.

Relevant references

1. Flatt, P.M., et al., p53-dependent expression of PIG3 during proliferation, genotoxic stress, and reversible growth arrest. Cancer Lett. 2000, 156, 63-73_x000B__x000B_2. Venot, C., et al., The requirement for the p53 proline-rich functional domain for mediation of apoptosis is correlated with specific PIG3 gene transactivation and with transcriptional repression. EMBO J. 1998, 17, 4668-4679_x000B__x000B_3. el-Deiry, W.S., Regulation of p53 downstream genes. Semin. Cancer Biol. 1998, 8, 345-357

 

About

Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.

 

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Antibody's suited for

Detects recombinant PIG-3 protein by Western blot. Optimal concentration should be evaluated by serial dilutions.

 

Other description

Provided as solution in phosphate buffered saline with 0.08% sodium azide. Ammonium Sulfate Precipitation

Antibody come from

Antibody developed using full-length recombinant PIG-3 protein.

 

Antibody's reacts with these species

This antibody doesn't cross react with other species

Antigen-antibody binding interaction

Rabbit anti PIG3 (p53 inducible gene 3) Antibody

 

Latin name

Oryctolagus cuniculus

Category

Primary Antibodies

 

Antibody's specificity

No Data Available

Application

Western Blot

 

Clone

Polyclonal

Protein number

see ncbi

 

Storage

-20ºC

Antibody is raised in

Rabbit

 

Antibody's reacts with

Human