Anti-p53 (FL-393) (Polyclonal), ALEXA Fluor 594

The best quality, top Anti-p53 (FL-393) (Polyclonal), ALEXA Fluor 594 prices, fast delivery.

Product price

489 EUR

Product size

100 microliters

Product catalog no.

GENTObs-8687R-A594

Fast and secure purchase process

Get on Gentaur.com

Background information

p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

 

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

 

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Synonyms

LFS1; p53; p53 Cellular Tumor Antigen; p53 Tumor Suppressor; Phosphoprotein p53; TP53; Transformation related protein 53; TRP53; Tumor protein p53; Tumour Protein p53.

 

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

 

Antigen Source

KLH conjugated synthetic peptide derived from human p53 (FL-393)

Specificity

This antibody reacts specifically with p53 (FL-393)

 

Modification

No modification has been applied to this antibody

Properties

For facs or microscopy Alexa 1 conjugate.

 

Also known as

p53 (FL-393) Polyclonal Antibody

Host organism

Rabbit (Oryctolagus cuniculus)

 

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Other name

Anti-p53 (FL-393) Polyclonal

 

Type

Conjugated Primary Antibody

Group

Polyclonals and antibodies

 

Purification method

Purified by Protein A.

Clone

Polyclonal Antibodies

 

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

 

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

 

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

 

Target Protein/Peptide

p53 FL-393

Applications

IF(IHC-P)

 

Modification site

None

Gene ID

7157

 

Swiss Prot

N/A

Subcellular locations

N/A