p53 Antibody ( BP5312)

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Product price

320 EUR

Product size

0,1 mg

Product catalog no.

MA1078

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Background

The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.

Related articles

1. Isobe, M.; Emanuel, B. S.; Givol, D.; Oren, M.; Croce, C. M. : Localization of gene for human p53 tumour antigen to band 17p13. Nature 320: 84-85, 1986. 2. Casey, G.; Yamanaka, Y.; Freiss, H.; Kobrin, M. S.; Lopez, M. E.; Buchler, M.; Beger, H. G.; Korc, M. : p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis. Cancer Lett. 69: 151-160, 1993.

 

Storage condtions

Keep the p53 Antibody ( BP5312)at minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.

Tips

The p53 Antibody ( BP5312) did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.

 

Properties

If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Synonyms

LFS1|TRP53|Li-Fraumeni syndrome|TRANSFORMATION-RELATED PROTEIN 53|Antigen NY-CO-13|Phosphoprotein p53|Tumor suppressor p53

 

Solubilization

The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.

Product configuration

Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

 

Uniprot ID

Human(P04637), Mouse(P02340), Rat(P10361)

Immunogen

Recombinant human wild-type p53 protein.

 

Entrez GeneID

Human(7157), Mouse(22059), Rat(24842)

Protein Name

Cellular tumor antigen p53

 

Type of the antibody

IgG2a monoclonal antibody

Reacts with species:

Human, chicken, mouse

 

Clonality

Monoclonal antibody

Analyses

WB,IHC-P,IHC-F,ICC

 

Gene Full Name

tumor protein p53

Product form

freeze-dried

 

French translation

anticorps

Purification

Ascites

 

Clone

BP53-12

Gene Name

TP53

 

Raised in

mice

Target antigen

p53