anti-Phospho-p53 Antibody

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Product price

480 EUR

Product size

Phospho-p53 Antibody

Product catalog no.

3515-100

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Background information:

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. p53 can apparently be phosphorylated by ATM, ATR, and DNA-PK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage.

Recommended usage:

Western blot analysis (0.5-4 µg/ml), immunoprecipitation (5-10 µg/ml), and Immunohistochemistry (20 µg/ml). However, the optimal conditions should be determined individually. The Phospho-p53 (Ser15) antibody detects ~53 kDa Ser15 phosphorylated p53. It does not react with non-phosphorylated p53 or p53 phosphorylated at other sites.

 

Formulation of this antibody:

100 µg (0.5 mg/ml) affinity purified rabbit polyclonal phospho-p53 (Ser15) antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

 

Properties

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Alternative names of antibody target:

TP53 , p53 , LFS1 , TRP53 , P53 , tumor protein p53 , Li-Fraumeni syndrome

 

Immunogene sequence:

Synthetic phospho-peptide surrounding amino acid Ser15 of human p53

How to handle this antibody:

The antibody solution should be gently mixed before use.

 

This antibody has been succesfully tested for use in following methods:

Western blot, Immunoprecipitation, Immunohistochemistry

Storage information:

Shelf time: 12 months in temp.: -20 deg.C

 

This antibody reacts with:

Human, mouse, rat

Purification method:

Affinity purified

 

Target of this antibody:

Phospho-p53

Isotype:

Rabbit IgG

 

Clonality of antibody:

Polyclonal

Concentration of antibody:

0.5 mg/ml

 

French translation

anticorps

Shipping conditions:

gel pack

 

Form of antibody:

Liquid

Antibody host:

Rabbit