MOUSE Anti-p53 (aa20-25):FITC Antibody

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Product price

486 EUR

Product size

100ug

Product catalog no.

GEN210753

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Properties

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.

Specificity and cross-reactivity

p53 This item recognises the human p53 tumour suppressor protein, binding to both wild type and mutant forms. p53 is important in multicellular organisms, where it regulates the cell cycle and thus functions as a tumor suppressor that is involved in preventing cancer. DO-1 recognises an epitope at the N-terminal end of p53 between amino acids 20-25.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

 

Storage and shipping

Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost free freezers is not recommended. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

 

Other names

cellular tumor antigen p53 isoform a; Cellular tumor antigen p53; cellular tumor antigen p53; antigen NY-CO-13; phosphoprotein p53; p53 tumor suppressor; transformation-related protein 53; tumor protein p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

Species reactivity

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

 

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Form/Appearance

FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)

 

Concentration

IgG concentration 0.1 miligram per 1 mililiter

Other gene names

TP53; TP53; P53; BCC7; LFS1; TRP53; P53

 

Tested applications:

Flow Cytometry (FACS)

Subcategory

Mnoclonal antibodies

 

Host organism

Mouse (Mus musculus)

Conjugation

Anti-FITC Antibody

 

Latin name

Mus musculus

Clonality

Monoclonal

 

Category

Antibodies

French translation

anticorps

 

Immunoglobulin isotype

IgG2a

Clone

DO-1

 

Purification method

N/A

Gene name synonims

N/A

 

Gene name

p53

Also known as

p53