The best Anti-p53 Antibody, quick delivery, high quality.
Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. P53 is subject to modification by conjugation of SUMO-1. A p53 mutant deficient for MDM2 binding is poorly sumoylated in vivo compared to wild-type p53. Overexpression of MDM2 increases the level of p53 sumoylation, which is further stimulated by expression of ARF. These results show that p53 sumoylation is regulated by MDM2- and ARF-mediated nucleolar targeting.
Aliquot the anti-p53 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Titration of the anti-p53 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 364-393 from the human protein was used as the immunogen for this anti-p53 antibody.
This anti-p53 antibodyis to be used only for research purposes and not for diagnostics..
IHC (Paraffin): 1:100,Western blot: 1:1000
In 1X PBS, pH 7.4, with 0.09% sodium azide
Rabbit (Oryctolagus cuniculus)
Polyclonal (rabbit origin)
IHC, WB, ELISA