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Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Human (Homo sapiens). Others not known. ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Flow Cytometry: 0.5-1ug/million cells in 0.1ml,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 minutes at RT
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
The optimal dilution of the p53 antibody for each application should be determined by the researcher.
Store the p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
This p53 antibodyis to be used only for research purposes and not for diagnostics..
Recombinant human p53 protein was used as the immunogen for this p53 antibody.
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Purified by protein A Affinity Chromatography
Rabbit (Oryctolagus cuniculus)
Polyclonal (rabbit origin)
FACS, IF, WB, IHC-P