p53 (FL-393) Antibody, FITC Conjugated

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Product price

333 EUR

Product size

0.1ml

Product catalog no.

bs-8687R-FITC

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Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.

Antigen background

p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

 

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Synonyms

LFS1; p53; p53 Cellular Tumor Antigen; p53 Tumor Suppressor; Phosphoprotein p53; TP53; Transformation related protein 53; TRP53; Tumor protein p53; Tumour Protein p53.

 

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Specificity

This is a highly specific antibody against p53 FL-393.

 

Long name

p53 (FL-393) Polyclonal Antibody, FITC Conjugated

Purification method

This antibody was purified via Protein A.

 

Host Organism

Rabbit (Oryctolagus cuniculus)

Source

Full length human p53 protein

 

Category

Conjugated Primary Antibodies

Clone

Polyclonal antibody

 

Recommended dilutions

IF(IHC-P)(1:50-200)

Additional conjugation

Anti-FITC Antibody

 

Crossreactivity

Human, Mouse, Rat

Excitation Emission

494nm/518nm

 

Concentration

1ug per 1ul

Modification

Unmodified

 

Clonality

Polyclonal

Target Antigen

p53 FL-393

 

French translation

anticorps

Tested applications

IF(IHC-P)

 

Modification site

None

Conjugation

FITC

 

Gene ID number

7157