P53(Ser392) Antibody, FITC Conjugated

The best P53(Ser392) Antibody, FITC Conjugated, quick delivery, high quality.

Product price

350 EUR

Product size

0.1ml

Product catalog no.

bs-1650R-FITC

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Antigen background

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.

 

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Synonyms

P53; BCC7; LFS1; TRP53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53

 

Source

KLH conjugated synthetic phosphopeptide derived from human p53 around the phosphorylation site of (Ser392)

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

 

Specificity

This is a highly specific antibody against P53 Ser392.

Long name

P53(Ser392) Polyclonal Antibody, FITC Conjugated

 

Purification method

This antibody was purified via Protein A.

Host Organism

Rabbit (Oryctolagus cuniculus)

 

Category

Conjugated Primary Antibodies

Clone

Polyclonal antibody

 

Recommended dilutions

IF(IHC-P)(1:50-200)

Additional conjugation

Anti-FITC Antibody

 

Modification

Phosphorylation

Immunogen_range

382-393/393

 

Excitation Emission

494nm/518nm

Concentration

1ug per 1ul

 

Clonality

Polyclonal

Target Antigen

P53 Ser392

 

French translation

anticorps

Tested applications

IF(IHC-P)

 

Swiss Prot

P04637

Modification site

Ser392

 

Crossreactivity

Human

Conjugation

FITC

 

Gene ID number

7157