P53 protein(wt-p53) Antibody, FITC Conjugated

The best quality, top P53 protein(wt-p53) Antibody, FITC Conjugated prices, fast delivery.

Product price

333 EUR

Product size

0.1ml

Product catalog no.

bs-0033R-FITC

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Antigen background

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.

 

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Synonyms

P53; BCC7; LFS1; TRP53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53

 

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Specificity

This is a highly specific antibody against P53 proteinwt-p53.

 

Long name

P53 protein(wt-p53) Polyclonal Antibody, FITC Conjugated

Source

KLH conjugated synthetic peptide derived from human P53

 

Purification method

This antibody was purified via Protein A.

Recommended dilutions

IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

 

Host Organism

Rabbit (Oryctolagus cuniculus)

Category

Conjugated Primary Antibodies

 

Clone

Polyclonal antibody

Subcellular location

Cytoplasm, Nucleus

 

Tested applications

IF(IHC-P), IF(ICC)

Additional conjugation

Anti-FITC Antibody

 

Target Antigen

P53 proteinwt-p53

Crossreactivity

Human, Mouse, Rat

 

Concentration

1ug per 1ul

Excitation Emission

494nm/518nm

 

Immunogen_range

240-290/393

Modification

Unmodified

 

Clonality

Polyclonal

French translation

anticorps

 

Swiss Prot

P04637

Modification site

None

 

Conjugation

FITC

Gene ID number

7157