P53(Ser315) Antibody, Biotin Conjugated

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Product price

350 EUR

Product size

0.1ml

Product catalog no.

bs-3704R-Biotin

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Antigen background

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

 

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Synonyms

P53; BCC7; LFS1; TRP53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53

 

Source

KLH conjugated synthetic phosphopeptide derived from human P53 around the phosphorylation site of Ser315

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

 

Specificity

This is a highly specific antibody against P53 Ser315.

Long name

P53(Ser315) Polyclonal Antibody, Biotin Conjugated

 

Purification method

This antibody was purified via Protein A.

Recommended dilutions

WB(1:100-1000), IHC-P(1:100-500)

 

Host Organism

Rabbit (Oryctolagus cuniculus)

Category

Conjugated Primary Antibodies

 

Clone

Polyclonal antibody

Subcellular location

Cytoplasm, Nucleus

 

Modification

Phosphorylation

Additional conjugation

Biotinylated

 

Immunogen_range

280-330/393

Concentration

1ug per 1ul

 

Clonality

Polyclonal

Target Antigen

P53 Ser315

 

French translation

anticorps

Tested applications

WB, IHC-P

 

Swiss Prot

P04637

Modification site

Ser315

 

Conjugation

Biotin

Crossreactivity

Human

 

Gene ID number

7157

Excitation Emission

N/A