p53 (FL-393) Antibody, Cy3 Conjugated

The best p53 (FL-393) Antibody, Cy3 Conjugated, quick delivery, high quality.

Product price

350 EUR

Product size

0.1ml

Product catalog no.

bs-8687R-Cy3

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Antigen background

p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-p53 FL-393 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.

 

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Synonyms

LFS1; p53; p53 Cellular Tumor Antigen; p53 Tumor Suppressor; Phosphoprotein p53; TP53; Transformation related protein 53; TRP53; Tumor protein p53; Tumour Protein p53.

 

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Specificity

This is a highly specific antibody against p53 FL-393.

 

Long name

p53 (FL-393) Polyclonal Antibody, Cy3 Conjugated

Purification method

This antibody was purified via Protein A.

 

Host Organism

Rabbit (Oryctolagus cuniculus)

Source

Full length human p53 protein

 

Category

Conjugated Primary Antibodies

Clone

Polyclonal antibody

 

Excitation Emission

512,550nm/570,615nm

Recommended dilutions

IF(IHC-P)(1:50-200)

 

Additional conjugation

cy3 conjugation kit

Crossreactivity

Human, Mouse, Rat

 

Concentration

1ug per 1ul

Modification

Unmodified

 

Clonality

Polyclonal

Target Antigen

p53 FL-393

 

French translation

anticorps

Tested applications

IF(IHC-P)

 

Modification site

None

Gene ID number

7157

 

Conjugation

Cy3