p53 (phospho-S33) polyclonal antibody

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Product price

448 EUR

Product size

100ul

Product catalog no.

BS4146-100ul

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About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Usage

Western blot (WB) analysis of p-p53 (S33) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with UV(4h) Lane2:Raw264.7 cell lysate treated with UV(4h) Lane3:PC12 cell lysate treated with UV(4h)

 

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

 

Specificity

p-p53 (S33) polyclonal antibody detects endogenous levels of p53 protein when phosphorylated at Ser33.

Alternative Name

Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53

 

Immunogen

Synthetic phosphopeptide derived from human p53 around the phosphorylation site of Serine 33.

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

 

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Note

For research use only, not for use in diagnostic procedure.

 

Group

Polyclonals and antibodies

Application_all

WB: 1:500~1:1000

 

Reactivity

Human,Mouse,Rat

BiowMW

~ 53,43 kDa

 

French translation

anticorps

Swiss-Prot

P04637

 

Applications

WB