P53(Ser20) Antibody, ALEXA FLUOR 647

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Product price

350 EUR

Product size

100ul

Product catalog no.

bs-3703R-A647

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Background of the antigen

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Primary Conjugated Antibodies. ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-P53 Ser20 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

 

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Synonyms

P53; BCC7; LFS1; TRP53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53

 

Source

KLH conjugated synthetic phosphopeptide derived from human P53 around the phosphorylation site of Ser20

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

 

Long name

P53(Ser20) Polyclonal Antibody, ALEXA FLUOR 647 Conjugated

Specificity

This is a highly specific antibody against P53 Ser20.

 

Also known as

Anti-P53 Ser20 PAb ALEXA FLUOR 647

Host Organism

Rabbit (Oryctolagus cuniculus)

 

Category

Conjugated Primary Antibodies

Conjugation

Alexa Fluor,ALEXA FLUOR 647

 

Purification

Purified by Protein A.

Recommended dilutions

IF(IHC-P)(1:50-200)

 

Clone

Polyclonal antibody

Subcellular location

Cytoplasm, Nucleus

 

Crossreactivity

Human, Mouse, Rat

Conjugated with

ALEXA FLUOR® 647

 

Modification

Phosphorylation

Excitation emission

650nm/665nm

 

Concentration

1ug per 1ul

Clonality

Polyclonal

 

Tested applications

IF(IHC-P)

Target Antigen

P53 Ser20

 

French translation

anticorps

Immunogen range

5-55/393

 

Swiss Prot

P04637

Modification Site

Ser20

 

Gene ID Number

7157