P53 protein(wt-p53) Antibody, ALEXA FLUOR 488

The best P53 protein(wt-p53) Antibody, ALEXA FLUOR 488, quick delivery, high quality.

Product price

380 EUR

Product size

100ul

Product catalog no.

bs-0033R-A488

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Background of the antigen

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-P53 proteinwt-p53 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

 

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Synonyms

P53; BCC7; LFS1; TRP53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53

 

Source

This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human P53

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

 

Long name

P53 protein(wt-p53) Polyclonal Antibody, ALEXA FLUOR 488 Conjugated

Specificity

This is a highly specific antibody against P53 proteinwt-p53.

 

Also known as

Anti-P53 proteinwt-p53 PAb ALEXA FLUOR 488

Recommended dilutions

IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

 

Host Organism

Rabbit (Oryctolagus cuniculus)

Category

Conjugated Primary Antibodies

 

Purification

Purified by Protein A.

Clone

Polyclonal antibody

 

Subcellular location

Cytoplasm, Nucleus

Tested applications

IF(IHC-P), IF(ICC)

 

Conjugated with

ALEXA FLUOR® 488

Crossreactivity

Human, Mouse, Rat

 

Target Antigen

P53 proteinwt-p53

Conjugation

Alexa Fluor

 

Concentration

1ug per 1ul

Immunogen range

240-290/393

 

Excitation emission

499nm/519nm

Modification

Unmodified

 

Clonality

Polyclonal

French translation

anticorps

 

Swiss Prot

P04637

Modification Site

None

 

Gene ID Number

7157