Rabbit Anti Mouse Tp53 Antibody-Biotin

The best Rabbit Anti Mouse Tp53 Antibody-Biotin, quick delivery, high quality.

Product price

302 EUR

Product size

100ug

Product catalog no.

CSB-PA024077LD01MO_100ug

Fast and secure purchase process

Get on Gentaur.com

Background

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression (By similarity). Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis, but seems to have to effect on cell-cycle regulation.

Properties

If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

 

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

About

Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

 

Shipping and storage

Tp53 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.

 

Buffer composition

Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Immunogen type

Recombinant mouse Cellular tumor antigen p53 protein (1-387AA)

 

Long name

Rabbit Antibody against Mouse Tp53, Biotin

Alias

Tumor suppressor p53,P53, Trp53,Tp53

 

Latin name

Mus musculus,Oryctolagus cuniculus

Short name of the antigen

Cellular tumor antigen p53

 

Format

Liquid, diluted in buffer

Antibody category

Polyclonal Antibodies

 

Synonym name

Tp53 Mouse Antibody

Clonality

Polyclonal antibody

 

Purification method

Protein G purified

Additional conjugation

Biotinylated

 

French translation

anticorps

UniProt ID

P02340

 

Conjugation

Biotin

Tested applications & recommended dilutions

ELISA

 

Species reactivity

Mouse

Immunogen species

Mouse

 

Purity

>95%

Antibody isotype

IgG