Quick contact, quick delivery, greatests Mouse Monoclonal IgG2b Anti-p53 Protein [DO-7] prices.
413 EUR
1 ml
PP100
1. Cattoretti, G., Rilke F., Andreola S., D'Amato L., Delia D. (1988) p53 expression in breast cancer. Int. J. Cancer 41; 178-183. 2. Nigro M.J., Baker S.J., Preisinger A.C., Jessup J.M., Hostetter R., Cleary K., et al. (1989) Mutations in the p53 gene occur in divers human tumor types. Nature 342; 705-708. 3. Voitesek, Bartek J., Midgley C.A., Lane D.P. (1992) An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53. J. Immunol. Methods 151; 237-244. 4. Van den Berg F.M., Baas I.O., Polak M.M., Offerhaus G.J.A. (1993) Detection of p53 overexpression in routinely paraffin-embedded tissue of human carcinomas using a novel target unmasking fluid. Am. J. Pathol. 142; 381-385.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Pre-treatment with Unmasking Fluid G (Art. No. DE007) or citrate buffer (Art. No. DE000) at 96-100°C.
N-terminal sequence of the human p53 protein, wild-type and mutant
Recombinant human p53 protein (wild type) produced in E. coli
Please contact us to check the availability
Go to UniProt/SwissProt
liquid concentrate
Breast carcinoma
Primary Antibody
Store at 2-8°C
Mus musculus
50 µg/ml
1:20-1:50
IHC(P)
IgG2b
Mouse
Human
DO-7