The best PIG3 (p53 inducible gene 3) - ELISA Kit, quick delivery, high quality.
532 EUR
1Each
X1326K1
1. Herrmann C, et al: Major vault protein of electric ray is a phosphoprotein. Neurochem Res 1998 Jan;23(1):39-46._x000B_2. Hamill DR, Suprenant KA: Characterization of the sea urchin major vault protein: a possible role for vault ribonucleoprotein particles in nucleocytoplasmic transport. Dev Biol 1997;190(1):117-128._x000B_3. Izquierdo MA, et al: Broad distribution of the multidrug resistance-related vault lung resistance protein in normal human tissues and tumors. Am J Pathol 1996 Mar;148(3):877-887._x000B_4. Hart SM, et al.: Expression of the human major vault protein LRP in acute myeloid leukemia. Exp Hematol 1997 Nov;25(12):1227-1232. _x000B_5. den Boer ML, et al: Relationship between major vault protein/lung resistance protein, multidrug resistance-associated protein, P-glycoprotein expression, and drug resistance in childhood leukemia. Blood 1998 Mar 15;91(6):2092-2098._x000B_6. Izquierdo MA, et al: Major vault protein LRP-related multidrug resistance. Eur J Cancer 1996 Jun;32A(6):979-984. _x000B_7. Schroeijers AB, et al.: Immunohistochemical detection of the human major vault protein LRP with two monoclonal antibodies in formalin-fixed, paraffin-embedded tissues. Am J Pathol 1998 Feb;152(2):373-378._x000B_8. Abbondanza C, et al: Interaction of vault particles with estrogen receptor in the MCF-7 breast cancer cell. J Cell Biol 1998;141(6):1301-1310._x000B_9. Dingemans AM, et al: Expression of the human major vault protein LRP in human lung cancer samples and normal lung tissues. Ann Oncol 1996 Aug;7(6):625-630. _x000B_10. Den Boer, M.L. et al. Relatiomship between major vault protein/lung resistance protein, multidrug resistance-associated protein, P-glycoprotein expression, and drug resistance in childhood leukemia. Blood 91, 2092-2098 (1998)._x000B_
The PIG3 ELISA is a sandwich enzyme immunoassay employing mouse monoclonal antibodies. A monoclonal antibody, specific for the human PIG3 protein, has been immobilized onto the surface of microtiter wells provided in the kit. The sample to be assayed and the biotinylated detector monoclonal antibody are pipetted into the wells and allowed to incubate for two hours, during which time any PIG3 present binds to the capture and detecting antibodies. Unbound material is washed away and horseradish peroxidase-conjugated streptavidin is added, which binds to the detector antibody._x000B_The horseradish peroxidase catalyzes the conversion of the chromogenic substrate tetra-methylbenzidine (TMB) from a colorless solution to a blue solution (or yellow after the addition of stop reagent), the intensity of which is proportional to the amount of PIG3 protein in the sample. The colored reaction product is quantified using a spectrophotometer._x000B_Quantitation is achieved by the construction of a standard curve using known concentrations_x000B_of PIG3 (provided lyophilized). By comparing the absorbance obtained from a sample containing an unknown amount of PIG3 with that obtained from the standards, the concentration of PIG3 in the sample can be determined.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
This antibody can be used for gel supershift assays, flow cytometry, immunoprecipitation (2 µg/mg of protein lysate), Western blotting (1 µg/ml) or immunohistochemistry on frozen or formalin/paraffin fixed tissues (2-4 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Hybridoma produced by the fusion of splenocytes from mice immunized with immunoprecipitated protein from phosphocellulose column flow through of extract of human breast cancer MCF-7 cells and mouse myeloma Sp2/0-Ag14 cells.
cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Provided as solution in phosphate buffered saline with 0.08% sodium azide.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
This antibody doesn't cross react with other species
PIG3 (p53 inducible gene 3) - ELISA Kit
No Data Available
Other Reagents
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see ncbi
-20ºC
ELISA
Human