Anti-P53(Ser315) (Polyclonal), ALEXA Fluor 594

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Product price

516 EUR

Product size

100 microliters

Product catalog no.

GENTObs-3704R-A594

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Background information

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

 

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

 

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Synonyms

P53; BCC7; LFS1; TRP53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53

 

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Antigen Source

KLH conjugated synthetic phosphopeptide derived from human P53 around the phosphorylation site of Ser315

 

Specificity

This antibody reacts specifically with P53(Ser315)

Properties

For facs or microscopy Alexa 1 conjugate.

 

Also known as

P53(Ser315) Polyclonal Antibody

Host organism

Rabbit (Oryctolagus cuniculus)

 

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Other name

Anti-P53(Ser315) Polyclonal

 

Type

Conjugated Primary Antibody

Group

Polyclonals and antibodies

 

Purification method

Purified by Protein A.

Clone

Polyclonal Antibodies

 

Cross reactive species

Human (Homo sapiens)

Clonality

Polyclonal Antibody

 

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Subcellular locations

Cytoplasm, Nucleus

 

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

 

Modification

Phosphorylation

Excitation emission

590nm/617nm

 

Concentration

1ug per 1ul

Target Protein/Peptide

P53 Ser315

 

Applications

IF(IHC-P)

Swiss Prot

P04637

 

Modification site

Ser315

Gene ID

7157