p53 Antibody (pSer37): FITC

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Product price

484 EUR

Product size

100 µl

Product catalog no.

SPC-1412D-FITC

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Properties

If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.

Alternative Name(s)

p53 tumor suppressor Antibody, Cellular tumor antigen p53 Antibody, Antigen NY-CO-13 Antibody, Phosphoprotein p53 Antibody, Tumor protein 53 Antibody, BCC7 Antibody, p53 Antibody, TP53 Antibody, P53_HUMAN Antibody, FLJ92943 Antibody, Mutant tumor protein 53 Antibody, Tumor protein p53 Antibody, LFS1 Antibody, Transformation related protein 53 Antibody, TRP53 Antibody, Tumor suppressor p53 Antibody

 

Cellular Localization

Mitochondrion Matrix, Endoplasmic Reticulum, Nucleus Pml Body, Nucleus, Pml Body, Cytoplasm, Cytosol, Mitochondrion, Nuclear Chromatin, Nuclear Matrix, Nucleolus, Nucleoplasm, Intracellular, Protein Complex, Replication Fork

Research Area(s)

Cancer, Cell Cycle, p53 Pathway, Cancer, Tumor suppressors, Apoptosis, Cell Signaling, Epigenetics and Nuclear Signaling, DNA/RNA, DNA Damage and Repair

 

Antibody Dilution

WB (1:250-1:1000); IHC (1:50-1:150); ELISA (1:5000); optimal dilutions for assays should be determined by the user.

Specificity

Detects endogenous levels of p53 protein only when phosphorylated at serine 37 (pSer37). Detects a band at ~44 kDa.

 

Immunogen

Synthetic phospho-peptide from human p53 around the phosphorylation site of serine 37 (pSer37).

Storage Buffer

PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide

 

Field of Use

For in vitro research use only.

Purification

Peptide Affinity Purified

 

Conjugation

Anti-FITC Antibody

Shipping Temperature

Blue Ice or 4ºC

 

Applications

WB, IHC, ELISA

Target

p53 (pSer37)

 

Accession Number

NP_000537.3

Product Type

Polyclonal

 

Category

Antibodies

French translation

anticorps

 

Storage Temperature

-20ºC

Host Species

Rabbit

 

Swiss-Prot

P04637

Immunogen Species

Human

 

Species Reactivity

Human

Conjugate

FITC

 

Gene ID

7157

Gross Weight (kg)

1.4

 

Net Weight (g)

0.1