Quick contact, quick delivery, greatests Human P53 PicoKine ELISA Kit prices.
623 EUR
1x96 well plate
EK0895
GENTAUR's human P53 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for P53 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, M1-D393) and your samples are measured with a biotin conjugated polyclonal from goat specific for P53 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human P53 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Catalog number;Description;Quantity -;96-well plate precoated with anti- human P53 antibodies;1 ST0000-10;Lyophilized recombinant human P53 standard;10ng/tube×2 AR1107;Biotinylated anti- human P53 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
P53(also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kilodalton(kDa) protein on SDS-PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full-length p53. It has played a vital role in conserving stability by preventing genome mutation.
Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human P53 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell lysate to the ELISA plate. Keep in mind that each human P53 standard solution and each sample be measured in duplicate.
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);1328;2789;5434;1502;2891;6120 ELISA standard deviations 78.4;173;353.21;103.7;214;508 CV(%);5.9;6.2;6.5;6.9;7.4;8.3
Antigen NY-CO-13;BCC7;Cellular tumor antigen p53;LFS1;MS980;p53;p53 tumor suppressor;P53_HUMAN;Phosphoprotein p53;Tp53;Transformation related protein 53;TRP53;Tumor suppressor p53
Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.028 + 0.071 + 0.120 + 0.225 + 0.365 + 0.664 + 1.283 + 2.001
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
the ELISA does not cross react with any other proteins except from closely related species
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
For quantitative measurement of human P53 in cell lysate.
Natural and recombinant human P53
Cellular tumor antigen p53
156pg/ml to 10000pg/ml
tumor protein p53
NSO, M1-D393
Human P53
< 10pg/ml
P04637
25-30
7157
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