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After reconstitution, the p53 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
The p53 tumor antigen induces cell cycle arrest or apoptosis in response to sub-lethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions.
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Titration of the pAb may be required due to differences in protocols and secondary/substrate sensitivity.
Human partial recombinant protein (AA 74-393) was used as the immunogen for this p53 antibody.
This p53 pAbis to be used only for research purposes and not for diagnostics..
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
Rabbit (Oryctolagus cuniculus)
Polyclonal (rabbit origin)
Antigen affinity purified