P53(Thr81) Antibody, FITC Conjugated

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Product price

350 EUR

Product size

0.1ml

Product catalog no.

bs-3711R-FITC

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Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.

Antigen background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013].

 

Synonyms

p53 Thr81; P53 phospho T81; P53 phospho Thr81; p-P53 Thr81; p-P53 T81; Widespread p53; Wtp53; Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; HGNC11998; LFS1; p53; p53 Cellular Tumor Antigen; p53 Tumor Suppressor; Phosphoprotein p53; TP53; Transformation related protein 53; TRP53; Tumor protein p53; Tumour Protein p53; P53_HUMAN; TP53.

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

 

Source

KLH conjugated synthetic phosphopeptide derived from human P53 around the phosphorylation site of Thr81

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

 

Specificity

This is a highly specific antibody against P53 Thr81.

Long name

P53(Thr81) Polyclonal Antibody, FITC Conjugated

 

Purification method

This antibody was purified via Protein A.

Host Organism

Rabbit (Oryctolagus cuniculus)

 

Category

Conjugated Primary Antibodies

Recommended dilutions

IF(IHC-P)(1:50-200)

 

Clone

Polyclonal antibody

Additional conjugation

Anti-FITC Antibody

 

Modification

Phosphorylation

Crossreactivity

Human, Mouse

 

Concentration

1ug per 1ul

Excitation Emission

494nm/518nm

 

Clonality

Polyclonal

French translation

anticorps

 

Tested applications

IF(IHC-P)

Target Antigen

P53 Thr81

 

Modification site

Thr81

Conjugation

FITC

 

Gene ID number

7157