p53 (Acetyl-K370) Rabbit monoclonal antibody

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Product price

408 EUR

Product size

50ul

Product catalog no.

BS9864M-50ul

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About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by Bioworld. Rabbit antibodies are very stable and can be stored for several days at room temperature. Bioworld adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Usage

Western blot (WB) analysis of p53 (Acetyl-K370) Rabbit mAb at 1:1000 dilution Lane 1: HepG2 treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours whole cell lysates Lane 2: HepG2 untreated whole cell lysates

 

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

N terminal acetylation or CH3CO as epigenetic regulation of p53 ( -K370) Rabbit monoclonal antibody by NATs.

 

Alternative Name

Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

 

Specificity

This antibody detects endogenous levels of p53 protein only when acetylated at Lys370.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

 

Note

For research use only, not for use in diagnostic procedure.

Application_all

WB: 1:1000-1:2000 ICC/IF: 1:50-1:200

 

Purification&Purity

Protein A affinity purified

Immunogen

Recombinant antibody.

 

Latin name

Oryctolagus cuniculus

Reactivity

Human,Mouse,Rat

 

BiowMW

~ 53, 43 kDa

Applications

WB ICC/IF

 

French translation

anticorps

Swiss-Prot

P04637