anti-TP53 Antibody, Monoclonal

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Product price

180 EUR

Product size

0.05 mg

Product catalog no.

49-495

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Gene info

The p53 gene like the Rb gene, is a tumor suppressor gene, i.e., its activity stops the formation of tumors. If a person inherits only one functional copy of the p53 gene from their parents, they are pre-disposed to cancer and usually develop several independent tumors in a variety of tissues in early adulthood. This condition is rare, and is known as Li-Fraumeni syndrome. However, mutations in p53 are found in most tumor types, and so contribute to the complex network of molecular events leading to tumor formation. In the cell, p53 protein binds DNA, which in turn stimulates another gene to produce a protein called p21 that interacts with a cell division-stimulating protein (cdk2). When p21 is complexed with cdk2 the cell cannot pass through to the next stage of cell division. Mutant p53 can no longer bind DNA in an effective way, and as a consequence the p21 protein is not made available to act as the 'stop signal' for cell division. Thus cells divide uncontrollably, and form tumors. p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. The amount of information that exists on all aspects of p53 normal function and mutant expression in human cancers is now vast, reflecting its key role in the pathogenesis of human cancers. It is clear that p53 is just one component of a network of events that culminate in tumor formation.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

 

Antigen

TP53 monoclonal antibody was made against amino acids 1 - 534 of TP53 (Human) using recombinant human p53 protein. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma P3-X63/AG8.653 cells using conventional hybridoma technology.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

 

Gene names

TP53, BCC7, Cellular tumor antigen p53, p53, TRP53, p53 tumor suppressor, Phosphoprotein p53, Antigen NY-CO-13, Tumor protein p53, Tumor suppressor p53, LFS1

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

 

Antibody advice

TP53 antibody can be used in ELISA starting at 1:1000, and immunohistochemistry starting at 10 ug/mL.

Keep at

Store TP53 antibody at 4 grades C or -20 grades C. As with all antibodies avoid freeze/thaw cycles.

 

Suspention, pH, azide

0.02 M potassium phosphate, 0.5 M sodium chloride, pH 7.2, 0.01% sodium azide.

Immuno application

ELISA, Western Blot, immuno-histo-chemistry, immuno precipitation

 

Antigene name

tumor protein p53

Antibodie's label

unlabelled serum

 

Purity

Protein A Column

Source

Homo sapiens

 

Vial with antibody

in solution

Type

Monoclonal

 

French translation

anticorps

Omim nr

269849759

 

Immunoglobulin

IgG2a,k

Ncbi nr

P04637

 

Protein nr

P04637

Ab raised in

Mouse

 

Works with

Human

GENE symbol

TP53

 

NCBI GENE nr

7157